Research publications

Our R&D team, apart from testing and developing products using herbs, also feels that, there must be validated research findings published in International journals as we never compromise in conducting quality research. As a result of our interest and focus towards quality, perfection and standards, we have published two research articles, one has been accepted and more articles are under review.

Our research publications includes,

i. World Journal of Pharmaceutical Sciences

Phytochemical screening and bioassay of Cassia alata leaf extract to study its skin hyperpigmentation activity

Abstract

Medicinal plants of Indian origin play a significant role in treating a broad spectrum of human diseases. With the increase in awareness about the side effects associated with the modern medicines, natural products seem a reliable alternate that’s considered safe and effective. The present study investigates the phytochemical screening and analysis of Cassia alata leaf extract against hyperpigmentation of skin due to spray allergy and under eye darkness. The present study reveals the efficacy of hydro-ethanolic extract of Cassia alata applied to under eye dark circles and hyper pigmented under arms due to regular usage of body spray. The results obtained are highly promising on regular application twice a day for 20 days. Visible difference is observed within one week of application and normal skin color is obtained within a month’s time.

ii. World Journal of Pharmaceutical Sciences

Cassia alata and Cassia auriculata – Review of their bioactive potential

Abstract

Nature has been a powerful source of powerful medicines for thousands of years and number of modern drugs has been extracted and exploited from natural sources, for its use in traditional medicine. Traditional herbal medicines have a long history of use and are generally considered to be safer than synthetic drugs. Over 50% of all modern clinical drugs are natural products that play an important role in drug development in pharmaceutical industries. The present communication constitutes a review on the distribution, phytography, medicinal properties and pharmacological actions of Cassia alata and Cassia auriculata. These plants are known to contain various active principles of therapeutic value and to possess biological activity against a number of diseases.

Keywords: Phytography, medicinal properties, pharmacological actions, Cassia alata, Cassia auriculata.

iii. Asian Journal of Pharmaceutical and Clinical Research

Evaluation of in vitro antibacterial activity of Cassia auriculata L. Roxb flower and leaf in different solvents. (Accepted, in press)

Abstract

Objective: To study the antibacterial efficacy of the crude extracts of Cassia auriculata flower and leaf prepared with various solvents like hexane, chloroform, acetone, ethyl acetate, Ethanol, n-butanol, and 50% hydro-ethanol and to determine its Minimum Inhibitory Concentration(MIC).

Methods: The extracts were tested against human pathogens [Staphylococcus aureus (MTCC3169), Escherichia coli (MTCC 728), Pseudomonas aeruginosa (MTCC741), Proteus vulgaris (MTCC7299), Klebsiella pneumoniae (MTCC661)] by agar well diffusion method. The percentage of viable cells was calculated and the minimum inhibitory concentration was determined by turbidimetric assay.

Results: The crude ethanolic extract of Cassia auriculata flower and leaf exhibited intense anti-bacterial activity against E. coli, S. aureus, P. aeruginosa and no inhibition against K. pneumoniae and P. vulgaris.

Conclusion: Frequent administration of antibiotics has led to emergence of various antibiotic resistant bacterial strains and hence herbal remedies are at the limelight to nullify bacterial infections. This study revealed the promising efficacy of Cassia auriculata flower and leaf extract prepared with acetone, ethanol, and 50% hydro ethanol to combat microbial infections.

iv. International Journal of pharmacy and biological science

In vitro study to evaluate the Antifungal potential of Cassia alata leaf extract against mold allergens (Under review)

Abstract

Cassia alata is an important medicinal plant that has diverse medicinal values. This study focused on the evaluation of phytochemical analysis, antifungal activity and identification of clinical isolate molds by ITS region sequencing. The fungal samples were collected from skin, infected patient wound and placed in Sabouraud Dextrose Broth at 25⁰C for 7-14 days. Individual colonies were identified by streak plate method, totally 24 organisms were isolated. Cassia alata leaves were collected, air dried, ground and extracted using hydro-ethanol in the ratio of 1:10. Antifungal assay was performed by well diffusion method. Out of the 24 fungal cultures, 2 cultures showed higher inhibition zone. The results obtained from ITS region sequencing, upon BLAST analysis showed maximum similarity Nigrospora species and Stilbella fimetaria. Thus, hydro-ethanolic leaf extract of Cassia alata can be used as a potential antifungal agent against Nigrospora species and Stilbella fimetaria.